Bowman-Birk inhibitor modifies transcription of autophagy and apoptosis genes in an in vitro model of Alzheimer's disorder.
Samaneh AkbariHassan AkramiAli MostafaeiSudabeh KianiPublished in: Journal of cellular biochemistry (2019)
Alzheimer, a current neurodegenerative disorder has adverse effects on memory and behavior. β-Amyloid peptide accumulations are the hallmarks of Alzheimer. Dysfunction of autophagy and apoptosis is detected in Alzheimer's disease. The effect of Bowman-Birk inhibitor (BBI), purified from soybean, was investigated in autophagy and apoptosis in Alzheimer treatment. Treated-PC12 cells with 1000 nM HgCl2 induced amyloid β (Aβ) accumulation. Treatment of PC12 cells with 1000 nM HgCl 2 and then 500 μg/mL BBI could decrease the expression ratio of Bax/Bcl2 and increase the expression of beclin1, Bnip3, Atg5, and autophagy-related genes. These results indicated that BBI could inhibit Aβ accumulation by inducing autophagy, and also the neuroprotective effect was detected through decreasing apoptosis in the in vitro model of Alzheimer's disease. These results provided further evidence for the potential effectiveness of BBI in the treatment of Alzheimer's disease.
Keyphrases
- endoplasmic reticulum stress
- oxidative stress
- cell death
- cognitive decline
- induced apoptosis
- cell cycle arrest
- signaling pathway
- poor prognosis
- diabetic rats
- photodynamic therapy
- randomized controlled trial
- combination therapy
- systematic review
- emergency department
- binding protein
- mass spectrometry
- mild cognitive impairment
- risk assessment
- working memory
- genome wide analysis
- long non coding rna
- atomic force microscopy