Protecting Effect of Bacillus coagulans T242 on HT-29 Cells Against AAPH-Induced Oxidative Damage.
Xiaoxi GaoYuhong ZhangGuangqing MuYunpeng XuXinmiao WangYanfeng TuoFang QianPublished in: Probiotics and antimicrobial proteins (2022)
The aim of the present study was to investigate the in vitro antioxidant potential of Bacillus coagulans T242. B. coagulans T242 showed better antioxidant activities, including the 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging ability, lipid peroxidation inhibiting ability and reducing ability, than those exerted by Lactobacillus rhamnosus GG (LGG). B. coagulans T242 positively regulated the expression of the nuclear factor erythroid 2-relatedfactor 2/Kelch-like ECH-associated protein-1 (Nrf2/Keap1) pathway-related proteins (Nrf2, Keap1, heine oxygenase-1 (HO-1)); increased antioxidant enzymes (glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD)); reduced the content of malondialdehyde (MDA) level; decreased the expression of inflammatory-related cytokines interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α); and thus increased the survival rate in 2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH)-damaged HT-29 cells. This study proved that B. coagulans T242 exerted antioxidative effects by quenching oxygen free radicals and activating the Nrf2 signaling pathway in HT-29 cells.
Keyphrases
- induced apoptosis
- oxidative stress
- signaling pathway
- cell cycle arrest
- nuclear factor
- pi k akt
- poor prognosis
- anti inflammatory
- rheumatoid arthritis
- diabetic rats
- endoplasmic reticulum stress
- hydrogen peroxide
- transcription factor
- binding protein
- ionic liquid
- cell proliferation
- bacillus subtilis
- high glucose
- fluorescent probe
- amyotrophic lateral sclerosis
- free survival
- energy transfer