Innovative and rapid antimicrobial susceptibility testing systems.
Alex van BelkumCarey-Ann D BurnhamJohn W A RossenFrederic MallardOlivier RochasWilliam Michael DunnePublished in: Nature reviews. Microbiology (2020)
Antimicrobial resistance (AMR) is a major threat to human health worldwide, and the rapid detection and quantification of resistance, combined with antimicrobial stewardship, are key interventions to combat the spread and emergence of AMR. Antimicrobial susceptibility testing (AST) systems are the collective set of diagnostic processes that facilitate the phenotypic and genotypic assessment of AMR and antibiotic susceptibility. Over the past 30 years, only a few high-throughput AST methods have been developed and widely implemented. By contrast, several studies have established proof of principle for various innovative AST methods, including both molecular-based and genome-based methods, which await clinical trials and regulatory review. In this Review, we discuss the current state of AST systems in the broadest technical, translational and implementation-related scope.
Keyphrases
- antimicrobial resistance
- human health
- loop mediated isothermal amplification
- high throughput
- clinical trial
- risk assessment
- healthcare
- primary care
- magnetic resonance
- physical activity
- randomized controlled trial
- transcription factor
- gene expression
- genome wide
- sensitive detection
- dna methylation
- single cell
- phase iii
- phase ii
- label free