Alterations of the Brain Proteome and Gut Microbiota in d-Galactose-Induced Brain-Aging Mice with Krill Oil Supplementation.
Qinqin JiangChenyang LuTingting SunJun ZhouYe LiTinghong MingLinquan BaiZaijie Jim WangXiuRong SuPublished in: Journal of agricultural and food chemistry (2019)
Brain aging is commonly associated with neurodegenerative disorders, but the ameliorative effect of krill oil and the underlying mechanism remain unclear. In this study, the components of krill oil were measured, and the antiaging effects of krill oil were investigated in mice with d-galactose (d-gal)-induced brain aging via proteomics and gut microbiota analysis. Krill oil treatment decreased the expression of truncated dopamine- and cAMP-regulated phosphoproteins and proteins involved in the calcium signaling pathway. In addition, the concentrations of dopamine were increased in the serum (p < 0.05) and brain (p > 0.05) due to the enhanced expressions of tyrosine-3-monooxygenase and aromatic l-amino acid decarboxylase. Moreover, krill oil alleviated gut microbiota dysbiosis, decreased the abundance of bacteria that consume the precursor tyrosine, and increased the abundance of Lactobacillus spp. and short-chain fatty acid producers. This study revealed the beneficial effect of krill oil against d-gal-induced brain aging and clarified the underlying mechanism through proteomics and gut microbiota analysis.
Keyphrases
- fatty acid
- resting state
- white matter
- functional connectivity
- signaling pathway
- high glucose
- diabetic rats
- amino acid
- cerebral ischemia
- mass spectrometry
- metabolic syndrome
- epithelial mesenchymal transition
- drug induced
- transcription factor
- oxidative stress
- poor prognosis
- single cell
- endothelial cells
- brain injury
- adipose tissue
- long non coding rna
- high resolution
- subarachnoid hemorrhage
- microbial community
- binding protein
- wild type
- smoking cessation