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Genomic deletions in Aureobasidium pullulans by an AMA1 plasmid for gRNA and CRISPR/Cas9 expression.

Audrey MasiKlara WögerbauerRobert L MachAstrid R Mach-Aigner
Published in: Fungal biology and biotechnology (2024)
The CRISPR/Cas9 transformation system presented in this study provides new opportunities for strain engineering of A. pullulans. This system allows expression of Cas9 and antibiotic resistance while being easy to adapt. This strategy could open the path to intensive genomic engineering in A. pullulans.
Keyphrases
  • crispr cas
  • genome editing
  • poor prognosis
  • copy number
  • binding protein
  • minimally invasive
  • escherichia coli
  • long non coding rna
  • gene expression
  • dna methylation