GNPS-guided discovery of xylacremolide C and D, evaluation of their putative biosynthetic origin and bioactivity studies of xylacremolide A and B.
Felix SchalkJanis FrickeSoohyun UmBenjamin H ConlonHannah MausNils JägerThorsten HeinzelTanja SchirmeisterMichael PoulsenChristine BeemelmannsPublished in: RSC advances (2021)
Targeted HRMS2-GNPS-based metabolomic analysis of Pseudoxylaria sp. X187, a fungal antagonist of the fungus-growing termite symbiosis, resulted in the identification of two lipopeptidic congeners of xylacremolides, named xylacremolide C and D, which are built from d-phenylalanine, l-proline and an acetyl-CoA starter unit elongated by four malonyl-CoA derived ketide units. The putative xya gene cluster was identified from a draft genome generated by Illumina and PacBio sequencing and RNAseq studies. Biological activities of xylacremolide A and B were evaluated and revealed weak histone deacetylase inhibitory (HDACi) and antifungal activities, as well as moderate protease inhibition activity across a panel of nine human, viral and bacterial proteases.
Keyphrases
- histone deacetylase
- single cell
- endothelial cells
- case control
- genome wide
- fatty acid
- small molecule
- sars cov
- candida albicans
- high throughput
- copy number
- high intensity
- induced pluripotent stem cells
- gene expression
- cancer therapy
- pluripotent stem cells
- drug delivery
- genome wide identification
- cell wall
- genome wide analysis