A new class of teraryl-based AIEgen for highly selective imaging of intracellular lipid droplets and its detection in advanced-stage human cervical cancer tissues.
Chandra Prakash SharmaAkanksha VyasPriyanka PandeyShashwat GuptaRavi Prakash VatsSakshi Priya JaiswalMadan Lal Brahma BhattMonika SachdevaAtul GoelPublished in: Journal of materials chemistry. B (2023)
Lipid droplets (LDs) have drawn much attention in recent years. They serve as the energy reservoir of cells and also play an important role in numerous physiological processes. Furthermore, LDs are found to be associated with several pathological conditions, including cancer and diabetes mellitus. Herein, we report a new class of teraryl-based donor-acceptor-appended aggregation-induced emission luminogen (AIEgen), 6a, for selective staining of intracellular LDs in in vitro live 3T3-L1 preadipocytes and the HeLa cancer cell line. In addition, AIEgen 6a was found to be capable of staining and quantifying the LD accumulation in the tissue sections of advanced-stage human cervical cancer patients. Unlike commercial LD staining dyes Nile Red, BODIPY and LipidTOX, AIEgen 6a showed a high Stokes shift (195 nm), a good fluorescence lifetime decay of 12.7 ns, and LD staining persisting for nearly two weeks.
Keyphrases
- endothelial cells
- papillary thyroid
- flow cytometry
- squamous cell
- induced pluripotent stem cells
- cell cycle arrest
- induced apoptosis
- high resolution
- pluripotent stem cells
- fluorescent probe
- reactive oxygen species
- fatty acid
- photodynamic therapy
- single molecule
- signaling pathway
- oxidative stress
- adipose tissue
- young adults
- cell proliferation