Prostate cancer reactivates developmental epigenomic programs during metastatic progression.
Mark M PomerantzXintao QiuYanyun ZhuDavid Y TakedaWenting PanSylvan C BacaAlexander GusevKeegan D KorthauerTesa M SeversonGavin HaSrinivas R ViswanathanJi-Heui SeoHolly M NguyenBaohui ZhangBogdan PasaniucClaudia GiambartolomeiSarah A AlaiwiConnor A BellEdward P O'ConnorMatthew S ChabotDavid R StillmanRosina LisAlba Font-TelloLewyn LiPaloma CejasAndries M BergmanJoyce SandersHenk G van der PoelSimon A GaytherKate LawrensonMarcos A S FonsecaJessica ReddyRosario I CoronaGleb MartovetskyBrian EganToni K ChoueiriLeigh EllisIsla P GarrawayGwo-Shu Mary LeeEva CoreyHenry W LongWilbert ZwartMatthew L FreedmanPublished in: Nature genetics (2020)
Epigenetic processes govern prostate cancer (PCa) biology, as evidenced by the dependency of PCa cells on the androgen receptor (AR), a prostate master transcription factor. We generated 268 epigenomic datasets spanning two state transitions-from normal prostate epithelium to localized PCa to metastases-in specimens derived from human tissue. We discovered that reprogrammed AR sites in metastatic PCa are not created de novo; rather, they are prepopulated by the transcription factors FOXA1 and HOXB13 in normal prostate epithelium. Reprogrammed regulatory elements commissioned in metastatic disease hijack latent developmental programs, accessing sites that are implicated in prostate organogenesis. Analysis of reactivated regulatory elements enabled the identification and functional validation of previously unknown metastasis-specific enhancers at HOXB13, FOXA1 and NKX3-1. Finally, we observed that prostate lineage-specific regulatory elements were strongly associated with PCa risk heritability and somatic mutation density. Examining prostate biology through an epigenomic lens is fundamental for understanding the mechanisms underlying tumor progression.
Keyphrases
- prostate cancer
- transcription factor
- radical prostatectomy
- benign prostatic hyperplasia
- squamous cell carcinoma
- small cell lung cancer
- gene expression
- public health
- dna methylation
- dna binding
- induced apoptosis
- cell death
- single cell
- oxidative stress
- rna seq
- genome wide
- genome wide identification
- long non coding rna