Visualization of spatial gene expression in plants by modified RNAscope fluorescent in situ hybridization.
Shyam SolankiGazala AmeenJin ZhaoJordan FlatenPawel BorowiczRobert S BrueggemanPublished in: Plant methods (2020)
RNAscope multiplex fluorescent RNA-ISH detection is well described and adapted for animal tissue samples, however due to morphological and structural differences in the plant tissues the standard protocol is deficient and required optimization. Utilizing barley specific HvGAPDH and Rpg1 RNA probes we report an optimized method which can be used for RNAscope detection to determine the spatial expression and semi-quantification of target RNAs. This optimized method will be immensely useful in other plant species such as the widely utilized Arabidopsis.
Keyphrases
- gene expression
- label free
- real time pcr
- living cells
- quantum dots
- loop mediated isothermal amplification
- poor prognosis
- dna methylation
- randomized controlled trial
- transcription factor
- nucleic acid
- small molecule
- high throughput
- fluorescent probe
- single molecule
- fluorescence imaging
- sensitive detection
- plant growth
- wild type