Login / Signup

Mapping and characterization of the Rx3 gene for resistance to Xanthomonas euvesicatoria pv. euvesicatoria race T1 in tomato.

Ge MengYao XiaoAitong LiZilin QianYinge XieLuyao YangHuabing LinWencai Yang
Published in: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik (2022)
) derived from a cross between Ohio 88119 and Ohio 9834, the Rx3 locus was finally mapped to a 64.3-kb interval between markers MG-Rx3-4 and MG-Rx3-A6. The Solyc05g053980 gene, designated as Rx3, encoding a coiled-coil nucleotide-binding leucine-rich repeat protein was considered as the candidate for the Rx3 locus. Expression of the gene could be induced by the infection of race T1 strain. Knockout of the Solyc05g053980 gene through CRISPR/Cas9 editing system in the resistant line FG02-7530 decreased resistance to race T1 strain. These results provide a close step for understanding the resistance mechanism to race T1 in Hawaii 7998 and guide tomato breeders accordingly to improve bacterial spot disease resistance in tomato.
Keyphrases
  • crispr cas
  • copy number
  • genome wide
  • genome editing
  • genome wide identification
  • poor prognosis
  • gene expression
  • high resolution
  • binding protein
  • small molecule
  • genome wide analysis
  • protein protein