Expression of a recombinant, 4'-Phosphopantetheinylated, active M. tuberculosis fatty acid synthase I in E. coli.
Szilvia BaronYoav PelegJacob GrunwaldDavid MorgensternNadav EladMoshe PeretzShira AlbeckYishai LevinJohn T WelchKim A DeWeerdAlon SchwarzYigal BursteinRon DiskinZippora ShakkedOren ZimhonyPublished in: PloS one (2018)
Purified hexameric and active Mtb FAS I is required for binding and drug inhibition studies and for structure-function analysis of this enzyme. This relatively simple and short procedure for Mtb FAS I production should facilitate studies of this enzyme.