PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions.
Xiaona HuangChao WeiFenjie LiLumeng JiaPengguihang ZengJiahe LiJin TanTuanfeng SunShaoshuai JiangJia WangXiuxiao TangQingquan ZhaoBin LiuLimin RongCheng LiJunjun DingPublished in: Protein & cell (2019)
Polycomb group (PcG) ring finger protein 6 (PCGF6), though known as a member of the transcription-repressing complexes, PcG, also has activation function in regulating pluripotency gene expression. However, the mechanism underlying the activation function of PCGF6 is poorly understood. Here, we found that PCGF6 co-localizes to gene activation regions along with pluripotency factors such as OCT4. In addition, PCGF6 was recruited to a subset of the super-enhancer (SE) regions upstream of cell cycle-associated genes by OCT4, and increased their expression. By combining with promoter capture Hi-C data, we found that PCGF6 activates cell cycle genes by regulating SE-promoter interactions via 3D chromatin. Our findings highlight a novel mechanism of PcG protein in regulating pluripotency, and provide a research basis for the therapeutic application of pluripotent stem cells.
Keyphrases
- cell cycle
- transcription factor
- gene expression
- genome wide identification
- genome wide
- dna methylation
- cell proliferation
- binding protein
- embryonic stem cells
- stem cells
- cell fate
- poor prognosis
- optical coherence tomography
- pluripotent stem cells
- dna damage
- genome wide analysis
- diabetic retinopathy
- copy number
- amino acid
- machine learning
- long non coding rna
- optic nerve
- bone marrow
- artificial intelligence
- deep learning