Generation, Characterization, and Application of Inducible Proliferative Adult Human Epicardium-Derived Cells.
Yang GeAnke M SmitsJia LiuJuan ZhangThomas J van BrakelMarie-José T H GoumansMonique R M JongbloedAntoine A F de VriesPublished in: Cells (2021)
Using an inducible LT expression system, inducible proliferative adult human EPDCs were generated displaying high proliferative capacity in the presence of doxycycline. These iEPDCs maintain essential epicardial characteristics with respect to morphology, EMT ability, and paracrine signaling following doxycycline removal. This renders iEPDCs a highly useful new in vitro model for studying human epicardial properties.