Hydrodynamic Injection for Developing NASH Model.
Haichuan WangXin ChenPublished in: Methods in molecular biology (Clifton, N.J.) (2022)
The hydrodynamic tail vein injection (HTVi) is a technique that is used to deliver plasmid genes into live mice or rats. The HTVi leads to the in vivo transfection of exogenous DNA primarily in the liver, serving as a reliable approach of establishing animal models for the study of liver diseases. The nonalcoholic steatohepatitis (NASH) is liver inflammation and damage resulting from an accumulation of fat in the liver. With the rising prevalence of obesity worldwide, NASH is becoming an increasingly common health problem. The pathogenesis of NASH is a multi-step process involving complicated pathways. The molecular mechanisms of NASH remain poorly understood. Here, we describe the use of HTVi to establish animal models for the study of NASH.
Keyphrases
- oxidative stress
- healthcare
- escherichia coli
- metabolic syndrome
- public health
- type diabetes
- adipose tissue
- insulin resistance
- high fat diet induced
- ultrasound guided
- risk factors
- gene expression
- single molecule
- physical activity
- fatty acid
- skeletal muscle
- transcription factor
- social media
- health information
- climate change
- bioinformatics analysis
- circulating tumor cells