Macular pigment-enriched oil production from genome-edited microalgae.
Inhwa SongSunbin KimJongrae KimHyeonjun OhJunhwan JangSu Jin JeongKwangryul BaekWeon-Sun ShinSang Jun SimEonseon JinPublished in: Microbial cell factories (2022)
Our research showed that lipids and pigments are simultaneously induced in the dZA strain. Since dZAs are generated by introducing pre-assembled sgRNA and Cas9-protein into cells, antibiotic resistance genes or selective markers are not inserted into the genome of dZA, which is advantageous for applying dZA mutant to food. Therefore, the enriched macular pigment oil extracted from improved strains (dZAs) can be further applied to various food products and nutraceuticals.
Keyphrases
- antibiotic resistance genes
- crispr cas
- optical coherence tomography
- diabetic retinopathy
- anaerobic digestion
- fatty acid
- induced apoptosis
- microbial community
- genome editing
- wastewater treatment
- cell cycle arrest
- cataract surgery
- genome wide
- escherichia coli
- high glucose
- human health
- age related macular degeneration
- diabetic rats
- endoplasmic reticulum stress
- drug induced
- protein protein
- cell death
- signaling pathway
- small molecule
- binding protein