Discovery and Optimization of Potent, Selective, and Brain-Penetrant 1-Heteroaryl-1 H -Indazole LRRK2 Kinase Inhibitors for the Treatment of Parkinson's Disease.
David A CanditoVladimir SimovAnmol GulatiSolomon D KattarRyan W ChauBlair T LapointeJoey L MethotDuane E DeMongThomas H GrahamRavi KurukulasuriyaMitchell H KeylorLing TongGregori J MorrielloJohn J ActonBarbara PioWeiguo LiuJack D ScottMichael J ArdolinoTheodore A MartinotMatthew L MaddessXin YanHakan GunaydinRachel L PalteSpencer E McMinnLisa NogleHongshi YuEllen C MinnihanCharles A LesburgPing LiuJing SuLaxminarayan G HegdeLily Y MoyJanice D WoodhouseRobert FaltusTina XiongPaul CiaccioJennifer A PiesvauxKarin M OtteMatthew E KennedyDavid Jonathan BennettErin F DiMauroMatthew J FellSanthosh NeelamkavilHarold B WoodPeter H FullerJ Michael EllisPublished in: Journal of medicinal chemistry (2022)
Inhibition of leucine-rich repeat kinase 2 (LRRK2) kinase activity represents a genetically supported, chemically tractable, and potentially disease-modifying mechanism to treat Parkinson's disease. Herein, we describe the optimization of a novel series of potent, selective, central nervous system (CNS)-penetrant 1-heteroaryl-1 H -indazole type I (ATP competitive) LRRK2 inhibitors. Type I ATP-competitive kinase physicochemical properties were integrated with CNS drug-like properties through a combination of structure-based drug design and parallel medicinal chemistry enabled by sp 3 -sp 2 cross-coupling technologies. This resulted in the discovery of a unique sp 3 -rich spirocarbonitrile motif that imparted extraordinary potency, pharmacokinetics, and favorable CNS drug-like properties. The lead compound, 25 , demonstrated exceptional on-target potency in human peripheral blood mononuclear cells, excellent off-target kinase selectivity, and good brain exposure in rat, culminating in a low projected human dose and a pre-clinical safety profile that warranted advancement toward pre-clinical candidate enabling studies.