Vitamin D status and determinants in Indian children and adolescents: a multicentre study.
Anuradha V KhadilkarNeha KajaleChirantap OzaRashmi OkeKetan GondhalekarVivek PatwardhanVaman KhadilkarZulf MughalRaja PadidelaPublished in: Scientific reports (2022)
Studies performed on Indian children to assess vitamin-D status have been on small sample sizes, limited to specific geographical locations and used non-standard methods to measure 25(OH)D 3 . This multicentre study assessed 25(OH)D 3 concentrations from dried blood spots (DBS) in 5-18-year-old Indian children and adolescents using a standardized protocol and identified factors contributing towards vitamin D deficiency. Cross-sectional, observational school-based study was conducted by multi-stage stratified random sampling. A city and nearby village were selected from 6 Indian states covering wide geographical areas. Demography, anthropometry, body-composition, dietary-intakes and DBS samples were collected. 25(OH)D 3 was assessed from DBS using Liquid chromatography with tandem-mass spectrometry. Vitamin-D status was assessed in 2500 children; with additional data collected on a subset (n = 669) to assess predictors. Mean vitamin-D concentration was 45.8 ± 23.9 nmol/L, 36.8% of subjects had sufficient vitamin-D (> 50 nmol/L); rural subjects and boys had higher concentrations (p < 0.05). On regression analysis, younger age, female-gender, overweight and urban residence significantly contributed to deficiency. More than half the Indian children/adolescents were vitamin-D deficient or insufficient. Our study reinforces vitamin-D deficiency as a major public health problem and the need for supplementation, food fortification and educating the population as initiatives required to improve sufficiency status.
Keyphrases
- tandem mass spectrometry
- body composition
- liquid chromatography
- young adults
- public health
- cross sectional
- physical activity
- ultra high performance liquid chromatography
- deep brain stimulation
- mass spectrometry
- high performance liquid chromatography
- simultaneous determination
- mental health
- high resolution mass spectrometry
- resistance training
- randomized controlled trial
- high resolution
- bone mineral density
- solid phase extraction
- electronic health record
- body mass index
- big data
- data analysis
- case control