Identification of Targeted Central Genes (IGF1 and HMOX1) by Indirect Cold Physical Plasma in Human Melanocytes.
Reza VafaeeBabak ArjmandMaryam Hamzeloo-MoghadamZahra RazzaghiMostafa Rezaei TaviraniSina Rezaei TaviraniPublished in: Journal of lasers in medical sciences (2022)
Introduction: Cold physical plasma is a growing tool in medicine which is applied for the treatment of different cancers. In the present study, the gene profiles of human melanocytes exposed to indirect cold physical plasma versus control individuals are analyzed via protein-protein interaction (PPI) network analysis. Methods: The gene expression profiles were derived from Gene Expression Omnibus (GEO), and the significant differentially expressed genes (DEGs) were decoded via "Expression Atlas". PPI network analysis was applied to find the targeted central genes by indirect cold physical plasma. Results: The main connected component of the constructed network including 74 queried DEGs and 50 added first neighbors was analyzed. Considering degree value, betweenness centrality, closeness centrality, and stress, IGF1 and HMOX1 were introduced as the central nodes. Conclusion: The finding of this study indicates that the down-regulation of IGF1 and the up-regulation of HMOX are the prominent events in response to indirect cold physical plasma treatment at the cellular level. Detection of related biological terms via gene ontology is suggested.
Keyphrases
- network analysis
- genome wide
- genome wide identification
- protein protein
- physical activity
- bioinformatics analysis
- mental health
- gene expression
- endothelial cells
- dna methylation
- copy number
- binding protein
- small molecule
- pi k akt
- poor prognosis
- combination therapy
- cancer therapy
- young adults
- signaling pathway
- squamous cell carcinoma
- pluripotent stem cells
- induced pluripotent stem cells
- early stage
- radiation therapy
- smoking cessation
- drug delivery
- sentinel lymph node
- lymph node