Direct Detection and Quantification of Neoantigens.
Qing WangJacqueline DouglassMichael S HwangEmily Han-Chung HsiueBrian J MogMing ZhangNickolas PapadopoulosKenneth W KinzlerShibin ZhouBert VogelsteinPublished in: Cancer immunology research (2019)
Many immunotherapeutic approaches under development rely on T-cell recognition of cancer-derived peptides bound to human leukocyte antigen molecules on the cell surface. Direct experimental demonstration that such peptides are processed and bound is currently challenging. Here, we describe a method that meets this challenge. The method entailed an optimized immunoprecipitation protocol coupled with two-dimensional chromatography and mass spectrometry. The ability to detect and quantify minute amounts of predefined antigens should be useful both for basic research in tumor immunology and for the development of rationally designed cancer vaccines.
Keyphrases
- mass spectrometry
- papillary thyroid
- cell surface
- squamous cell
- randomized controlled trial
- high performance liquid chromatography
- lymph node metastasis
- dendritic cells
- high speed
- amino acid
- immune response
- childhood cancer
- peripheral blood
- label free
- induced pluripotent stem cells
- loop mediated isothermal amplification
- pluripotent stem cells