Targeted regulation of fibroblast state by CRISPR-mediated CEBPA expression.

Wei LiuJeffrey A MeridewAja AravamudhanGiovanni LigrestiDaniel J TschumperlinQi Tan

Published in: Respiratory research (2019)

CEBPA expression is reduced in human IPF fibroblasts and its deficiency reduces adipogenic potential and promotes fibrogenic activation. CEBPA expression can be rescued via an inhibitor of epigenetic repression or by targeted CRISPR activation, leading to reduced fibrogenic activation.

Keyphrases

poor prognosis
crispr cas
genome wide
genome editing
endothelial cells
dna methylation
binding protein
gene expression
long non coding rna
extracellular matrix
climate change
induced pluripotent stem cells
smoking cessation