Cytotoxic, Apoptosis-Inducing Activities, and Molecular Docking of a New Sterol from Bamboo Shoot Skin Phyllostachys heterocycla var. pubescens.
Reda F A AbdelhameedMohamed S NafieAhmed K IbrahimKoji YamadaMaged S Abdel-KaderAmany K IbrahimSafwat A AhmedJihan M BadrEman S HabibPublished in: Molecules (Basel, Switzerland) (2020)
Phytochemical screening of nonpolar fractions from the methanol extract of the Bamboo shoot skin Phyllostachys heterocycla var. pubescens resulted in the isolation of a new sterol-glucoside-fatty acid derivative (6'-O-octadeca-8'',11''-dienoyl)-sitosterol-3-O-β-d-glucoside (1), together with six known compounds. The chemical structures of the pure isolated compounds were deduced based on different spectral data. The isolated compounds were assessed to determine their cytotoxic activity, and the results were confirmed by determining their apoptotic activity. Compound 1 was more cytotoxic against the MCF-7 cells (IC50 = 25.8 µM) compared to Fluorouracil (5-FU) (26.98 µM), and it significantly stimulated apoptotic breast cancer cell death with 32.6-fold (16.63% compared to 0.51 for the control) at pre-G1 and G2/M-phase cell cycle arrest and blocked the progression of MCF-7 cells. Additionally, RT-PCR results further confirmed the apoptotic activity of compound 1 by the upregulation of proapoptotic genes (P53; Bax; and caspases 3, 8, and 9) and downregulation of the antiapoptotic genes (BCL2). Finally, the identified compounds, especially 1, were found to have high binding affinity towards both tyrosine-specific protein kinase (TPK) and vascular endothelial growth factor receptor (VEGFR-2) through the molecular docking studies that highlight its mode of action.
Keyphrases
- cell cycle arrest
- cell death
- molecular docking
- vascular endothelial growth factor
- molecular dynamics simulations
- fatty acid
- pi k akt
- signaling pathway
- cell proliferation
- breast cancer cells
- induced apoptosis
- genome wide
- endothelial cells
- soft tissue
- wound healing
- oxidative stress
- gene expression
- magnetic resonance imaging
- optical coherence tomography
- computed tomography
- high resolution
- electronic health record
- big data
- mass spectrometry
- case control