A high-resolution single-molecule sequencing-based Arabidopsis transcriptome using novel methods of Iso-seq analysis.
Runxuan ZhangRichard KuoMax CoulterCristiane P G CalixtoJuan Carlos EntizneWenbin GuoYamile MarquezLinda MilneStefan RieglerAkihiro MatsuiMaho TanakaSarah HarveyYubang GaoTheresa Wießner-KrohAlejandro PaniaguaMartin CrespiKatherine DenbyAsa Ben HurEnamul HuqMichael JantschArtur JarmolowskiTino KoesterSascha LaubingerQingshun Quinn LiLianfeng GuMotoaki SekiDorothee StaigerRamanjulu SunkarZofia Szweykowska-KulinskaShih-Long TuAndreas WachterRobbie WaughLiming XiongXiao-Ning ZhangAna ConesaAnireddy S N ReddyAndrea BartaMaria KalynaJohn W S BrownPublished in: Genome biology (2022)
AtRTD3 is the most comprehensive Arabidopsis transcriptome currently. It improves the precision of differential gene and transcript expression, differential alternative splicing, and transcription start/end site usage analysis from RNA-seq data. The novel methods for identifying accurate splice junctions and transcription start/end sites are widely applicable and will improve single-molecule sequencing analysis from any species.