Berberine protects against sepsis-related acute lung injury in rats via PPAR-γ signaling pathway upregulation and improvement at the cellular level: Functional, biochemical, and immunohistochemistry study.
Mohamed Mansour KhalifaNermeen A BastawyLaila A RashedHanan A HassanOmnia M Abdel-MaksoudFatma E HassanPublished in: General physiology and biophysics (2024)
This study aimed to assess the prophylactic effects of Berberine on experimentally induced lung sepsis and examine its effects on selected cytokines, genes, and protein expression besides the histopathological evaluation. Berberine significantly reduced the wet/dry lung ratio, the broncho-alveolar lavage fluid (BALF) protein, cells, neutrophils percentage, and cytokines levels. In addition, pretreatment with Berberine decreased the myeloperoxidase (MPO) and malondialdehyde (MDA) levels and decreased gene expression of nuclear factor kappa B (NF-κB), monocyte chemoattractant protein-1 (MCP-1), and the intracellular adhesion molecule 1 (ICAM-1) by RT-qPCR analysis, revealing Berberine's antioxidant and anti-inflammatory mode of action. Western blot analysis revealed increased peroxisome proliferator-activated receptor gamma (PPAR-γ) expression in the Berberine pretreated group compared to the cecal ligation and puncture (CLP) group, in which the histopathological examination evidenced this improvement. In conclusion, Berberine improved lung sepsis via its PPAR-γ mediated antioxidant and anti-inflammatory effects.
Keyphrases
- nuclear factor
- signaling pathway
- anti inflammatory
- gene expression
- toll like receptor
- acute kidney injury
- oxidative stress
- intensive care unit
- induced apoptosis
- poor prognosis
- insulin resistance
- binding protein
- pi k akt
- dna methylation
- lps induced
- dendritic cells
- fatty acid
- lipopolysaccharide induced
- endothelial cells
- skeletal muscle
- immune response
- high glucose
- inflammatory response
- peripheral blood
- south africa
- diabetic rats
- drug induced
- pseudomonas aeruginosa