MiR-548a-3p regulates inflammatory response via TLR4/NF-κB signaling pathway in rheumatoid arthritis.
Yingliang WangFeng ZhengGuohong GaoShushan YanLaixia ZhangLi WangXiao CaiXiaodong WangDonghua XuJibo WangPublished in: Journal of cellular biochemistry (2018)
Currently published studies have implicated that microRNAs (miRNAs) including exosomes-encapsulated miRNAs play a critical role in rheumatoid arthritis (RA). Previously, we have found that exosomes-encapsulated miR-548a-3p was significantly decreased in serum samples from RA patients by miRNAs microarray analysis. However, little is known of the role of miR-548a-3p in the development and progression of RA. In this study, we aim to investigate the underlying molecular mechanisms of miR-548a-3p in RA, which will provide new insight into understanding the pathogenesis of RA and identifying novel therapeutics targets for this disease. As validated by quantitative real-time polymerase chain reaction (qRT-PCR), the expression of miR-548a-3p in serum exosomes and peripheral blood mononuclear cells (PBMCs) of RA patients (n = 76) was obviously down-regulated compared with healthy controls (n = 20). Serum exosomal miR-548a-3p was negatively associated with levels of CRP, RF, and ESR in serum of patients with RA. MiR-548a-3p could inhibit the proliferation and activation of pTHP-1 cells by regulating the TLR4/NF-κB signaling pathway. Accordingly, exosomes-delivered miR-548a-3p may be a critical factor predicting the disease activity of RA. MiR-548a-3p/TLR4/NF-κB axis can serve as promising targets for RA diagnosis and treatment.
Keyphrases
- rheumatoid arthritis
- disease activity
- signaling pathway
- ankylosing spondylitis
- inflammatory response
- systemic lupus erythematosus
- rheumatoid arthritis patients
- mesenchymal stem cells
- induced apoptosis
- pi k akt
- lps induced
- end stage renal disease
- interstitial lung disease
- toll like receptor
- juvenile idiopathic arthritis
- ejection fraction
- nuclear factor
- stem cells
- newly diagnosed
- chronic kidney disease
- immune response
- oxidative stress
- prognostic factors
- high resolution
- bone marrow
- systematic review
- randomized controlled trial
- lipopolysaccharide induced
- transcription factor
- data analysis
- binding protein