Bio-engineered cell membrane nanovesicles as precision theranostics for perihilar cholangiocarcinoma.
Xiaojie ZhangYang ZhangYunming ZhangPeng LvPengfei ZhangChengchao ChuJingsong MaoXiaoyong WangWengang LiGang LiuPublished in: Biomaterials science (2020)
Perihilar cholangiocarcinoma (PHCC) presents a formidable challenge due to its occult anatomic location, aggressive growth, insensitivity to conventional chemotherapy, and poor prognosis. Herein, we engineered a human epidermal growth factor receptor 2 (HER2) affibody to the surface of cell membrane nanovesicles (A-NVs) in a ligand-oriented manner and loaded them with indocyanine green (ICG) as precision theranostics for PHCC treatment. The A-NVs@ICG were prepared and exhibited satisfactory targeting effects in HER2-overexpressing PHCC cells. In vivo fluorescence and photoacoustic imaging demonstrated that A-NVs@ICG promoted the accumulation of ICG in PHCC tissue, leading to enhanced tumor regression and improved anti-cancer effects when combined with photoirradiation. Therefore, bio-engineered A-NVs@ICG represent a promising nanotheranostic agent for PHCC with potential for clinical translation.
Keyphrases
- fluorescence imaging
- poor prognosis
- epidermal growth factor receptor
- photodynamic therapy
- long non coding rna
- tyrosine kinase
- induced apoptosis
- endothelial cells
- cancer therapy
- advanced non small cell lung cancer
- drug delivery
- cell cycle arrest
- endoplasmic reticulum stress
- cell death
- mass spectrometry
- squamous cell carcinoma
- quantum dots
- wound healing
- pluripotent stem cells