Local chromatin environment of a Polycomb target gene instructs its own epigenetic inheritance.
Scott BerryMatthew HartleyTjelvar S G OlssonCaroline DeanMartin HowardPublished in: eLife (2015)
Inheritance of gene expression states is fundamental for cells to 'remember' past events, such as environmental or developmental cues. The conserved Polycomb Repressive Complex 2 (PRC2) maintains epigenetic repression of many genes in animals and plants and modifies chromatin at its targets. Histones modified by PRC2 can be inherited through cell division. However, it remains unclear whether this inheritance can direct long-term memory of individual gene expression states (cis memory) or instead if local chromatin states are dictated by the concentrations of diffusible factors (trans memory). By monitoring the expression of two copies of the Arabidopsis Polycomb target gene FLOWERING LOCUS C (FLC) in the same plants, we show that one copy can be repressed while the other is active. Furthermore, this 'mixed' expression state is inherited through many cell divisions as plants develop. These data demonstrate that epigenetic memory of FLC expression is stored not in trans but in cis.
Keyphrases
- gene expression
- dna methylation
- genome wide
- poor prognosis
- transcription factor
- working memory
- mitochondrial dna
- copy number
- genome wide identification
- single cell
- dna damage
- cell therapy
- induced apoptosis
- binding protein
- long non coding rna
- oxidative stress
- signaling pathway
- cell cycle arrest
- deep learning
- mesenchymal stem cells
- cell death
- genome wide analysis
- cell wall