LncRNA MALAT1 from human adipose-derived stem cell exosomes accelerates wound healing via miR-378a/FGF2 axis.
Li PiLi YangBai-Rong FangXian-Xi MengLi QianPublished in: Regenerative medicine (2022)
Aim: The effects of MALAT1 from human adipose-derived stem cell (ADSC) exosomes in skin wound healing were investigated. Material & methods: The viability, apoptosis and migration ability of human skin fibroblasts (HSFs) were evaluated by Cell Counting Kit-8 assay, flow cytometry and scratch assay, respectively. A mouse model was established to evaluate the role of exosomal MALAT1 in skin wound healing in vivo . Results: Human ADSC exosomes promoted the proliferation and migration of HSFs and increased MALAT1 expression. MALAT1 silencing in human ADSCs inhibited HSF viability and migration, promoted HSF apoptosis and inhibited angiogenesis by upregulating miR-378a. Overexpression of miR-378a inhibited the migration and proliferation of HSFs by downregulating FGF2 expression. ADSC exosomes promoted skin wound healing by mediating MALAT1 in vivo . Conclusion: Exosomal MALAT1 accelerated skin wound healing by regulating the miR-378a/FGF2 axis, suggesting that MALAT1 might be used as a potential target for cutaneous wound treatment.
Keyphrases
- wound healing
- stem cells
- endothelial cells
- cell proliferation
- long non coding rna
- poor prognosis
- mesenchymal stem cells
- long noncoding rna
- mouse model
- induced pluripotent stem cells
- flow cytometry
- oxidative stress
- pluripotent stem cells
- endoplasmic reticulum stress
- signaling pathway
- cell therapy
- soft tissue
- single cell
- transcription factor
- binding protein
- cell cycle arrest
- mass spectrometry
- atomic force microscopy