Impact of In Vitro Passive Permeability in a P-gp-transfected LLC-PK1 Model on the Prediction of the Rat and Human Unbound Brain-to-Plasma Concentration Ratio.
Johan NicolaiHélène ChapyEric GillentKenneth SaundersAnna-Lena UngellJean-Marie NicolasHugues ChanteuxPublished in: Pharmaceutical research (2020)
We have succesfully validated a methodology to use a P-gp overexpressing LLC-PK1 cell line to predict both rat and human Kpuu,brain by correcting for both passive permeability and P-gp expression levels.