AXIN2 Reduces the Survival of Porcine Induced Pluripotent Stem Cells (piPSCs).
Rui ZhangShuai YuQiaoyan ShenWenxu ZhaoJuqing ZhangXiaolong WuZhenshuo ZhuXiaojie WuNa LiSha PengJinlian HuaPublished in: International journal of molecular sciences (2021)
The establishment of porcine pluripotent stem cells (piPSCs) is critical but remains challenging. All piPSCs are extremely sensitive to minor perturbations of culture conditions and signaling network. Inhibitors, such as CHIR99021 and XAV939 targeting the WNT signaling pathway, have been added in a culture medium to modify the cell regulatory network. However, potential side effects of inhibitors could confine the pluripotency and practicability of piPSCs. This study aimed to investigate the roles of AXIN, one component of the WNT pathway in piPSCs. Here, porcine AXIN1 and AXIN2 genes were knocked-down or overexpressed. Digital RNA-seq was performed to explore the mechanism of cell proliferation and apoptosis. We found that (1) overexpression of the porcine AXIN2 gene significantly reduced survival and negatively impacted the pluripotency of piPSCs, and (2) knockdown of AXIN2, a negative effector of the WNT signaling pathway, enhanced the expression of genes involved in cell cycle but reduced the expression of genes related to cell differentiation, death, and apoptosis.
Keyphrases
- cell proliferation
- cell cycle
- pi k akt
- rna seq
- signaling pathway
- cell cycle arrest
- single cell
- poor prognosis
- genome wide
- oxidative stress
- stem cells
- endoplasmic reticulum stress
- induced pluripotent stem cells
- genome wide identification
- cell death
- induced apoptosis
- epithelial mesenchymal transition
- immune response
- transcription factor
- regulatory t cells
- embryonic stem cells
- pluripotent stem cells
- cell therapy
- long non coding rna
- gene expression
- copy number
- free survival
- cancer therapy
- human health
- drug induced