Effects of Pulsed Electromagnetic Field Treatment on Skeletal Muscle Tissue Recovery in a Rat Model of Collagenase-Induced Tendinopathy: Results from a Proteome Analysis.
Enrica TorrettaManuela MoriggiDaniele CapitanioCarlotta Perucca OrfeiVincenzo RaffoStefania SettiRuggero CadossiLaura de GirolamoCecilia GelfiPublished in: International journal of molecular sciences (2024)
Tendon disorders often result in decreased muscle function and atrophy. Pulsed Electromagnetic Fields (PEMFs) have shown potential in improving tendon fiber structure and muscle recovery. However, the molecular effects of PEMF therapy on skeletal muscle, beyond conventional metrics like MRI or markers of muscle decline, remain largely unexplored. This study investigates the metabolic and structural changes in PEMF-treated muscle tissue using proteomics in a rat model of Achilles tendinopathy induced by collagenase. Sprague Dawley rats were unilaterally induced for tendinopathy with type I collagenase injection and exposed to PEMFs for 8 h/day. Gastrocnemius extracts from untreated or PEMF-treated rats were analyzed with LC-MS/MS, and proteomics differential analysis was conducted through label-free quantitation. PEMF-treated animals exhibited decreased glycolysis and increased LDHB expression, enhancing NAD signaling and ATP production, which boosted respiratory chain activity and fatty acid beta-oxidation. Antioxidant protein levels increased, controlling ROS production. PEMF therapy restored PGC1alpha and YAP levels, decreased by tendinopathy. Additionally, myosins regulating slow-twitch fibers and proteins involved in fiber alignment and force transmission increased, supporting muscle recovery and contractile function. Our findings show that PEMF treatment modulates NAD signaling and oxidative phosphorylation, aiding muscle recovery through the upregulation of YAP and PGC1alpha and increasing slow myosin isoforms, thus speeding up physiological recovery.
Keyphrases
- skeletal muscle
- insulin resistance
- label free
- rotator cuff
- mass spectrometry
- platelet rich plasma
- fatty acid
- poor prognosis
- high glucose
- oxidative stress
- type diabetes
- metabolic syndrome
- stem cells
- cell death
- mesenchymal stem cells
- signaling pathway
- computed tomography
- combination therapy
- ms ms
- risk assessment
- bone marrow
- endothelial cells
- replacement therapy