Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells.
Stephen D CarterCheri M HamptonRobert LangloisRoberto MeleroZachary J FarinoMichael J CalderonWen LiCallen T WallaceNgoc-Han T TranRobert A GrassucciStephanie E SiegmundJoshua G PembertonTravis J MorgensternLeanna EisenmanJenny I AguilarNili L GreenbergElana S LevyEdward YiWilliam G MitchellWilliam J RiceChristoph WiggeJyotsna PilliEmily W GeorgeDespoina AslanoglouMaïté CourelRobin J FreybergJonathan A JavitchZachary P WillsEstela Area-GomezSruti ShivaFrancesca BartoliniAllen VolchukSandra A MurrayMeir AridorKenneth N FishPeter WalterTamas BallaDeborah FassSharon Grayer WolfSimon C WatkinsJosé María CarazoGeorge J LuJoachim FrankZachary Z FreybergPublished in: Science advances (2020)
The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.
Keyphrases
- endoplasmic reticulum
- induced apoptosis
- high resolution
- cell cycle arrest
- single cell
- cell therapy
- oxidative stress
- endoplasmic reticulum stress
- spinal cord
- signaling pathway
- breast cancer cells
- high throughput
- single molecule
- transcription factor
- spinal cord injury
- photodynamic therapy
- high speed
- optical coherence tomography
- label free
- bone marrow