Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development.
Elaine MaiJoyce ChanLevina GoonBraeden K EgoJack BeversTiffany WongManda WongRacquel CorpuzHongkang XiJia WuKellen SchneiderDhaya SeshasayeeMichele GrimbaldestonGerald NakamuraVahan B IndjeianMenno van Lookeren CampagneKelly M LoyetLaetitia Comps-AgrarPublished in: Journal of translational medicine (2021)
Unlike existing commercial hIL-33 assays, our hIL-33 bioassays are highly sensitive and specific and can accurately quantify hIL-33 in various human clinical matrices, including those with high levels of hsST2. Our results provide a proof of concept of the utility of these assays in clinical trials targeting the hIL-33/hST2 pathway.
Keyphrases
- endothelial cells
- age related macular degeneration
- clinical trial
- high throughput
- induced pluripotent stem cells
- pluripotent stem cells
- high resolution
- randomized controlled trial
- cystic fibrosis
- cancer therapy
- mass spectrometry
- single cell
- air pollution
- phase ii
- fluorescent probe
- double blind
- simultaneous determination
- molecularly imprinted
- liquid chromatography
- placebo controlled