Cryopreservation of Human Mucosal Leukocytes.

Sean M HughesZhiquan ShuClaire N LevyApril L FerreHeather HartigCifeng FangGretchen LentzMichael FialkowAnna C KirbyKristina M Adams WaldorfRonald S VeazeyAnja GermannHagen von BriesenM Juliana McElrathCharlene S DezzuttiElizabeth SinclairChris A R BakerBarbara L ShacklettDayong GaoFlorian Hladik

Published in: PloS one (2016)

Leukocytes from cervicovaginal and colorectal tissues can be cryopreserved with good recovery of functional, viable cells using several different cryopreservation media. The number and volume of washes has an experimentally meaningful effect on the percentage of cells recovered. We provide a detailed, step-by-step protocol with best practices for cryopreservation of mucosal leukocytes.

Keyphrases

induced apoptosis
cell cycle arrest
peripheral blood
endothelial cells
randomized controlled trial
healthcare
primary care
gene expression
cell death
oxidative stress
mesenchymal stem cells
ulcerative colitis
bone marrow
induced pluripotent stem cells
pluripotent stem cells