A protocol for cloning, expression, and purification of Lysine exporter (LysE) gene of Mycobacterium tuberculosis.

Shilpa UpadhyayArchana DhokVinod AgarkarSupriya KashikarMahalaqua Nazli Khatib

Published in: F1000Research (2024)

Despite being a potential anti-tubercular target, research is quite limited on this protein. Therefore, we aim to purify LysE protein for further analysis. Similar protocols have already been implemented to purify several other bacterial proteins with >95% purity.

Keyphrases

mycobacterium tuberculosis
binding protein
amino acid
poor prognosis
protein protein
randomized controlled trial
genome wide
copy number
gene expression
small molecule
dna methylation
climate change