' Candidatus Liberibacter asiaticus'-Encoded BCP Peroxiredoxin Suppresses Lipopolysaccharide-Mediated Defense Signaling and Nitrosative Stress In Planta.
Mukesh JainLulu CaiIan BlackParastoo AzadiRussell W CarlsonKathryn M JonesDean W GabrielPublished in: Molecular plant-microbe interactions : MPMI (2022)
The lipopolysaccharides (LPS) of gram-negative bacteria trigger a nitrosative and oxidative burst in both animals and plants during pathogen invasion. Liberibacter crescens strain BT-1 is a surrogate for functional genomic studies of the uncultured pathogenic ' Candidatus Liberibacter' spp. that are associated with severe diseases such as citrus greening and potato zebra chip. Structural determination of L. crescens LPS revealed the presence of a very long chain fatty acid modification. L. crescens LPS pretreatment suppressed growth of X anthomonas perforans on nonhost tobacco ( Nicotiana benthamiana ) and X. citri subsp. citri on host orange ( Citrus sinensis ), confirming bioactivity of L. crescens LPS in activation of systemic acquired resistance (SAR). L. crescens LPS elicited a rapid burst of nitric oxide (NO) in suspension cultured tobacco cells. Pharmacological inhibitor assays confirmed that arginine-utilizing NO synthase (NOS) activity was the primary source of NO generation elicited by L. crescens LPS. LPS treatment also resulted in biological markers of NO-mediated SAR activation, including an increase in the glutathione pool, callose deposition, and activation of the salicylic acid and azelaic acid (AzA) signaling networks. Transient expression of ' Ca . L. asiaticus' bacterioferritin comigratory protein (BCP) peroxiredoxin in tobacco compromised AzA signaling, a prerequisite for LPS-triggered SAR. Western blot analyses revealed that ' Ca . L. asiaticus' BCP peroxiredoxin prevented peroxynitrite-mediated tyrosine nitration in tobacco. ' Ca . L. asiaticus' BCP peroxiredoxin (i) attenuates NO-mediated SAR signaling and (ii) scavenges peroxynitrite radicals, which would facilitate repetitive cycles of ' Ca . L. asiaticus' acquisition and transmission by fecund psyllids throughout the limited flush period in citrus.[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
Keyphrases
- inflammatory response
- anti inflammatory
- nitric oxide
- lps induced
- toll like receptor
- high frequency
- fatty acid
- poor prognosis
- single cell
- signaling pathway
- induced apoptosis
- south africa
- endothelial cells
- candida albicans
- gene expression
- high resolution
- mass spectrometry
- binding protein
- cell death
- endoplasmic reticulum stress
- long non coding rna
- amino acid
- genome wide
- cell proliferation
- fluorescent probe
- innate immune
- combination therapy
- sensitive detection
- human milk
- stress induced