Establishment of an efficient early flowering-assisted CRISPR/Cas9 gene-editing system in Arabidopsis.
Kangwen LaoYinlin XiaoQing HuangBeixin MoXianxin DongXiaoyan WangPublished in: Plant cell reports (2022)
Ectopic expression of the florigen FT gene along with the Cas9 cassette promotes fast reproduction of the T1 transgenic plants, and the late-flowering phenotype serves as a convenient marker for transgene-free plants screening in T2 segregation population.