Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants.

Liyang ZhangGen LiYingxiao ZhangYanhao ChengNathaniel RobertsSteve E GlennDiane DeZwaan-McCabeH Tomas RubeJeff MantheyGary ColemanChristopher A VakulskasYiping Qi

Published in: Genome biology (2023)

Our results demonstrate that LbCas12a-RVQ is a powerful tool for genome editing in human cells while LbCas12a-RRV confers robust genome editing in plants. Our study reveals the tremendous potential of these LbCas12a variants for advancing precision genome editing applications across a wide range of organisms.

Keyphrases

genome editing
crispr cas
copy number
gene expression
genome wide
multidrug resistant