Sophoridine Counteracts Obesity via Src-Mediated Inhibition of VEGFR Expression and PI3K/AKT Phosphorylation.
Jingchun SunXiaoting WangYulin HeXuekai TianTiantian YuanGong-She YangTaiyong YuPublished in: International journal of molecular sciences (2024)
Sophoridine (SRP) is a natural quinolizidine alkaloid found in many traditional Chinese herbs, though its effect on adipose tissue is unclear. We improved serum lipid levels by administering SRP by gavage in high-fat diet (HFD)-fed C57BL/6 mice. After 11 weeks, SRP supplementation significantly reduced body weight gain and improved glucose homeostasis, while reducing subcutaneous fat and liver weight. SRP also inhibited cell proliferation and differentiation of 3T3-L1 cells. Proteomics analysis revealed that SRP inhibits adipocyte differentiation by interacting with Src, thereby suppressing vascular endothelial growth factor receptor 2 (VEGFR2) expression and PI3K/AKT phosphorylation. This study provides an empirical basis for the treatment of obesity with small molecules.
Keyphrases
- pi k akt
- high fat diet
- weight gain
- adipose tissue
- insulin resistance
- vascular endothelial growth factor
- cell cycle arrest
- signaling pathway
- cell proliferation
- high fat diet induced
- induced apoptosis
- body mass index
- weight loss
- poor prognosis
- birth weight
- metabolic syndrome
- protein kinase
- tyrosine kinase
- cell death
- type diabetes
- skeletal muscle
- fatty acid
- binding protein
- endothelial cells
- mass spectrometry
- oxidative stress
- long non coding rna
- physical activity
- cell cycle
- endoplasmic reticulum stress
- blood pressure