miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages.
Zhenyu LuJing WangXu WangZhiying LiDan NiuMin WangJinzhu XiangYongli YueYajuan XiaXueling LiPublished in: Stem cells international (2021)
Human embryonic stem cells (hESCs) possess the ability to differentiate into insulin-producing cells (IPCs), which can be used to treat type I diabetes. miR-375 is an essential transcriptional regulator in the development and maturation of the pancreas. In this study, we optimized a protocol to differentiate hESCs into IPCs and successfully obtained IPCs. Then, we performed overexpression and inhibition experiments of miR-375 on cells at different stages of differentiation and performed RNA-seq. The results showed that the expression of miR-375 fluctuated during hESC differentiation and was affected by miR-375 mimics and inhibitors. miR-375 influences global gene expression and the target genes of miR-375. The overexpression of miR-375 can cause changes in multiple signaling pathways during pancreatic development. miR-375 is a major participant in the differentiation of pancreatic β-cells through different target genes at different stages. This study provides new ideas for further investigation of how microRNAs affect cell fate and gene transcription.
Keyphrases
- cell proliferation
- long non coding rna
- long noncoding rna
- gene expression
- induced apoptosis
- poor prognosis
- type diabetes
- genome wide
- transcription factor
- randomized controlled trial
- cardiovascular disease
- endothelial cells
- dna methylation
- cell cycle arrest
- skeletal muscle
- cell death
- glycemic control
- cell fate
- heat shock protein
- bioinformatics analysis
- induced pluripotent stem cells