An In Vivo CRISPR Screen Identifies Stepwise Genetic Dependencies of Metastatic Progression.

Manuel C ScheidmannFrancesc Castro GinerKarin StrittmatterIlona KrolAino Paasinen-SohnsRamona ScherrerCinzia DonatoSofia GkountelaBarbara M SzczerbaZoi DiamantopoulouSimone MuenstTatjana VlajnicLeo KunzMarcus VetterChristoph RochlitzVerdon TaylorClaudio GiachinoTimm SchroederRandall J PlattNicola Aceto

Published in: Cancer research (2022)

A loss-of-function CRISPR screen in human CTC-derived xenografts identifies genes critical for individual steps of the metastatic cascade, suggesting novel drivers and treatment opportunities for metastatic breast cancers.

Keyphrases

genome wide
dna methylation
squamous cell carcinoma
small cell lung cancer
copy number
high throughput
crispr cas
endothelial cells
genome editing
combination therapy
circulating tumor cells
pluripotent stem cells
circulating tumor