Expression profile of circular RNA in rat intimal hyperplasia and target gene prediction.
Tao LiZhi-Hua RongNeng-Bin ChangXing LiuJia-Ying XuDong LiuCong-Cong ShiWen-Yi ZhangRui JiangJiang JunPublished in: Journal of cellular physiology (2019)
Intimal hyperplasia is an important cause of stenosis or occlusion after vascular injury. Circular RNAs (circRNAs) are known to be related to various cardiovascular diseases. However, the expression profile of circRNAs in the neointima has not been reported in detail. In this study, we established a rat common carotid artery (CCA) injury model. A microarray detection showed significant differences in circRNA expression between the normal and injured CCA. Real-time quantitative polymerase chain reaction verified the differences. We used bioinformatics to predict the microRNAs that possibly interact with the differentially expressed (DE) circRNAs and linked the potential functions of circRNAs to the target genes of the microRNAs. We believe that the DE circRNA in neointima may affect the differentiation, proliferation, and migration of vascular cells through a variety of target genes. The intervention or utilization of certain circRNAs should be a new method for preventing and treating intimal hyperplasia.
Keyphrases
- genome wide
- genome wide identification
- cardiovascular disease
- bioinformatics analysis
- induced apoptosis
- oxidative stress
- poor prognosis
- randomized controlled trial
- smooth muscle
- genome wide analysis
- cell cycle arrest
- copy number
- type diabetes
- cell death
- coronary artery disease
- risk assessment
- mass spectrometry
- long non coding rna
- pi k akt
- cardiovascular risk factors