Proteomics of the heart.
Oleg A KarpovAleksandr StotlandKoen RaedscheldersBlandine ChazarinLizhuo AiChristopher I MurrayJennifer E Van EykPublished in: Physiological reviews (2024)
Mass spectrometry-based proteomics is a sophisticated identification tool specializing in portraying protein dynamics at a molecular level. Proteomics provides biologists with a snapshot of context-dependent protein and proteoform expression, structural conformations, dynamic turnover, and protein-protein interactions. Cardiac proteomics can offer a broader and deeper understanding of the molecular mechanisms that underscore cardiovascular disease, and it is foundational to the development of future therapeutic interventions. This review encapsulates the evolution, current technologies, and future perspectives of proteomic-based mass spectrometry as it applies to the study of the heart. Key technological advancements have allowed researchers to study proteomes at a single-cell level and employ robot-assisted automation systems for enhanced sample preparation techniques, and the increase in fidelity of the mass spectrometers has allowed for the unambiguous identification of numerous dynamic posttranslational modifications. Animal models of cardiovascular disease, ranging from early animal experiments to current sophisticated models of heart failure with preserved ejection fraction, have provided the tools to study a challenging organ in the laboratory. Further technological development will pave the way for the implementation of proteomics even closer within the clinical setting, allowing not only scientists but also patients to benefit from an understanding of protein interplay as it relates to cardiac disease physiology.
Keyphrases
- mass spectrometry
- cardiovascular disease
- robot assisted
- liquid chromatography
- heart failure
- label free
- single cell
- healthcare
- binding protein
- primary care
- end stage renal disease
- chronic kidney disease
- gas chromatography
- type diabetes
- coronary artery disease
- left ventricular
- atrial fibrillation
- physical activity
- poor prognosis
- protein protein
- minimally invasive
- high throughput
- single molecule
- cardiovascular risk factors
- tandem mass spectrometry
- postmenopausal women