Anti-Cancer Effects of Lactobacillus plantarum L-14 Cell-Free Extract on Human Malignant Melanoma A375 Cells.
Jaehyun ParkMijin KwonJaehoon LeeSangkyu ParkJeongmin SeoSangho RohPublished in: Molecules (Basel, Switzerland) (2020)
Human malignant melanoma is the most aggressive type of skin cancer with high metastatic ability. Despite several traditional therapies, the mortality rate remains high. Lactobacillus plantarum (L. plantarum), a species of lactic acid bacteria (LAB), is being studied for human health, including cancer treatment. However, few studies have elucidated the relationship between L. plantarum extract and human malignant melanoma. To investigate the effects of L. plantarum on human melanoma cells, A375 human melanoma cells were used and treated with L. plantarum L-14 extract. After the treatment, viability, migration ability, molecular changes of migration- and apoptosis-related genes, and the location of cytochrome c was evaluated. The L-14 extract inhibited the viability, migration of A375 cells as well as reduced expression of migration-related genes. In addition, it was confirmed that the L-14 extract induced intrinsic apoptosis in A375 cells. This study demonstrated that the L-14 extract exerted anticancer effects on A375 cells. Therefore, these data suggest that the L-14 extract is worth studying for the development of melanoma drugs using LAB.
Keyphrases
- cell cycle arrest
- oxidative stress
- induced apoptosis
- endothelial cells
- cell death
- induced pluripotent stem cells
- pluripotent stem cells
- anti inflammatory
- small cell lung cancer
- risk assessment
- cell free
- squamous cell carcinoma
- pi k akt
- skin cancer
- type diabetes
- poor prognosis
- cardiovascular disease
- climate change
- signaling pathway
- cardiovascular events
- deep learning
- single molecule
- electronic health record
- case control
- long non coding rna
- genetic diversity