A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection.
Michael M KaminskiMiguel A AlcantarIsadora T LapeRobert GreensmithAllison C HuskeJacqueline A ValeriFrancisco M MartyVerena KlämbtJamil AzziEnver AkalinLeonardo V RiellaJames J CollinsPublished in: Nature biomedical engineering (2020)
In organ transplantation, infection and rejection are major causes of graft loss. They are linked by the net state of immunosuppression. To diagnose and treat these conditions earlier, and to improve long-term patient outcomes, refined strategies for the monitoring of patients after graft transplantation are needed. Here, we show that a fast and inexpensive assay based on CRISPR-Cas13 accurately detects BK polyomavirus DNA and cytomegalovirus DNA from patient-derived blood and urine samples, as well as CXCL9 messenger RNA (a marker of graft rejection) at elevated levels in urine samples from patients experiencing acute kidney transplant rejection. The assay, which we adapted for lateral-flow readout, enables-via simple visualization-the post-transplantation monitoring of common opportunistic viral infections and of graft rejection, and should facilitate point-of-care post-transplantation monitoring.
Keyphrases
- crispr cas
- end stage renal disease
- ejection fraction
- chronic kidney disease
- newly diagnosed
- genome editing
- high throughput
- cell therapy
- prognostic factors
- peritoneal dialysis
- circulating tumor
- stem cells
- liver failure
- dna methylation
- extracorporeal membrane oxygenation
- epstein barr virus
- circulating tumor cells
- quantum dots
- mechanical ventilation
- label free
- nucleic acid
- acute respiratory distress syndrome
- single cell
- electron microscopy