Members of the nitronate monooxygenase gene family from Metarhizium brunneum are induced during the process of infection to Plutella xylostella.
Karla Yadira Cervantes QuinteroIsrael Enrique Padilla GuerreroJuan Carlos Torres-GuzmánBeatriz Guadalupe Villa MartínezAdelissa Valencia FélixGloria Angelica Gonzalez-HernandezPublished in: Applied microbiology and biotechnology (2020)
Metarhizium species are the most abundant fungi that can be isolated from soil, with a well-known biopesticide capacity. Metarhizium recognizes their hosts when the conidium interacts with insects, where the fungi are in contact with the hydrocarbons of the outermost lipid layer cuticle. These cuticular hydrocarbons comprise a mixture of n-alkanes, n-alkenes, and methyl-branched chains. Metarhizium can degrade insect hydrocarbons and use these hydrocarbons for energy production and the biosynthesis of cellular components. The metabolism of nitroalkanes involves nitronate monooxygenase activity. In this work, we isolated a family of six genes with potential nitronate monooxygenase activity from Metarhizium brunneum. The six genes were expressed in Escherichia coli, and the nitronate monooxygenase activity was verified in the recombinant proteins. Additionally, when the conidia of M. brunneum were grown in medium with nitroalkanes, virulence against Plutella xylostella increased. Furthermore, we analyzed the expression of the six Npd genes during the infection to this insect, which showed differential expression of the six Npd genes during infection.
Keyphrases
- escherichia coli
- genome wide
- bioinformatics analysis
- genome wide identification
- poor prognosis
- staphylococcus aureus
- pseudomonas aeruginosa
- binding protein
- dna methylation
- genome wide analysis
- risk assessment
- high glucose
- endothelial cells
- long non coding rna
- oxidative stress
- fatty acid
- antimicrobial resistance
- drug induced
- multidrug resistant