Evolutionary superscaffolding and chromosome anchoring to improve Anopheles genome assemblies.
Robert M WaterhouseSergey AganezovYoann AnselmettiJiyoung LeeLivio RuzzanteMaarten J M F ReijndersRomain FeronSèverine BérardPhillip GeorgeMatthew W HahnPaul I HowellMaryam KamaliSergey KorenDaniel LawsonGareth MaslenAshley PeeryAdam M PhillippyMaria V SharakhovaEric TannierMaria F UngerSimo V ZhangMax A AlekseyevNora J BesanskyCedric ChauveScott J EmrichIgor V SharakhovPublished in: BMC biology (2020)
Experimental data from probe mapping, RNAseq, or long-read technologies, where available, all contribute to successful upgrading of draft assemblies. Our evaluations show that gene synteny-based computational methods represent a valuable alternative or complementary approach. Our improved Anopheles reference assemblies highlight the utility of applying comparative genomics approaches to improve community genomic resources.