Radiation Responses of Human Mesenchymal Stem Cells Derived From Different Sources.
Ningning HeChangyan XiaoYuxiao SunYan WangLiqing DuYu FengYang LiuQin WangKaihua JiJinhan WangManman ZhangChang XuQiang LiuPublished in: Dose-response : a publication of International Hormesis Society (2019)
Mesenchymal stem cells (MSCs) derived from different tissues may aid in the regeneration of radiation-induced organ lesions; however, the radiation responses of human MSCs from different sources are unknown. In our study, a comparison of the results from cell proliferation, apoptosis, cell cycle, DNA damage, and DNA repair assays consistently showed that MSCs derived from adipose tissue possess a significantly stronger radiation resistance capacity than MSCs derived from umbilical cord and gingival, which is accompanied by a higher level of phosphorylated signal transducer and activator of transcription 3 (Stat3) expression. This reminds us Stat3 could be a potential biomarker of radiation resistance. These findings provide a better understanding of radiation-induced biologic responses in MSCs and may lead to the development of better strategies for stem cell treatment and cancer therapy.
Keyphrases
- radiation induced
- mesenchymal stem cells
- umbilical cord
- cell proliferation
- cell cycle
- dna repair
- dna damage
- stem cells
- radiation therapy
- endothelial cells
- adipose tissue
- bone marrow
- cancer therapy
- oxidative stress
- cell therapy
- poor prognosis
- drug delivery
- rheumatoid arthritis
- drinking water
- gene expression
- insulin resistance
- pluripotent stem cells
- transcription factor
- cell death
- high fat diet
- nuclear factor
- high throughput
- metabolic syndrome
- immune response
- endoplasmic reticulum stress
- single cell
- skeletal muscle
- replacement therapy