Organic washes of tissue sections for comprehensive analysis of small molecule metabolites by MALDI MS imaging of rat brain following status epilepticus.

Hui YangWenliang JiMing GuanShilei LiYangyang ZhangZhenwen ZhaoLanqun Mao

Published in: Metabolomics : Official journal of the Metabolomic Society (2018)

A simple organic washing protocol enables comprehensive analysis of tissue SMMs in MALDI MSI by removing ionization-suppressing lipids. The application in the SE model indicates that MALDI MSI analysis potentially provides new insight for understanding the disease mechanism.

Keyphrases

mass spectrometry
small molecule
gas chromatography
high resolution
liquid chromatography
ms ms
randomized controlled trial
signaling pathway
water soluble
multiple sclerosis
protein protein
fatty acid
tandem mass spectrometry
simultaneous determination