Generation of Sarcoplasmic and Myofibrillar Protein-Bound Heterocyclic Amines in Chemical Model Systems under Different Heating Temperatures and Durations.

The protein-bound heterocyclic amines (HAs) and their generation pattern are still unclear. Generation of sarcoplasmic (SP)- and myofibrillar protein (MP)-bound HAs under different heating conditions was investigated in chemical model systems using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The results showed that eight types (387.01 ± 37.50 ng/100 mg MP) and six types (452.06 ± 33.32 ng/100 mg SP) of protein-bound HAs were generated at 180 °C/40 min, respectively. MP system can generate either more types or amounts of bound HAs at 90-150 °C. The main categories of protein-bound HAs in two systems were almost the same: β-carbolines, α-carbolines, pyridines, and quinolines, among which nonpolar HAs dominated by β-carbolines (at least 69.2%) are most easily formed. Principal component analysis revealed no significant difference in bound HAs at 90-120 °C but showed a significant increase at 150-180 °C. The higher the temperature, the more significant the differences between samples with different durations at the same temperature.