An improved expression and purification protocol enables the structural characterization of Mnt1, an antifungal target from Candida albicans.

Patrícia Alves SilvaAmanda Araújo SouzaGideane Mendes de OliveiraMarcelo Henrique Soller RamadaNahúm Valente HernándezHéctor Manuel Mora-MontesRenata Vieira BuenoDiogo Martins-de-SaSonia Maria de FreitasMaria Sueli Soares FelipeJoão Alexandre Ribeiro Gonçalves Barbosa

Published in: Fungal biology and biotechnology (2024)

The methodology presented here can substantially improve the yield of recombinant CaMnt1 expressed in flask-grown yeasts. In addition, the structural characterization of the fungal mannosyltransferase presents novelties that can be exploited for new antifungal drug's development.

Keyphrases

candida albicans
biofilm formation
poor prognosis
randomized controlled trial
binding protein
saccharomyces cerevisiae
cell free
adverse drug
escherichia coli
plant growth
pseudomonas aeruginosa